Super-resolution optical fluctuation imaging

Super-resolution optical fluctuation imaging (SOFI) is a post-processing method for the calculation of super-resolved images from recorded image time series that is based on the temporal correlations of independently fluctuating fluorescent emitters. SOFI has been developed for super-resolution of biological specimen that are labelled with independently fluctuating fluorescent emitters (organic dyes, fluorescent proteins). In comparison to other super-resolution microscopy techniques such as STORM or PALM that rely on single-molecule localization and hence only allow one active molecule per diffraction-limited area (DLA) and timepoint, SOFI does not necessitate a controlled photoswitching and/ or photoactivation as well as long imaging times. Nevertheless, it still requires fluorophores that are cycling through two distinguishable states, either real on-/off-states or states with different fluorescence intensities. In mathematical terms SOFI-imaging relies on the calculation of cumulants, for what two distinguishable ways exist. For one thing an image can be calculated via auto-cumulants that by definition only rely on the information of each pixel itself, and for another thing an improved method utilizes the information of different pixels via the calculation of cross-cumulants. Both methods can increase the final image resolution significantly although the cumulant calculation has its limitations. Actually SOFI is able to increase the resolution in all three dimensions. == Principle == Likewise to other super-resolution methods SOFI is based on recording an image time series on a CCD- or CMOS camera. In contrary to other methods the recorded time series can be substantially shorter, since a precise localization of emitters is not required and therefore a larger quantity of activated fluorophores per diffraction-limited area is allowed. The pixel values of a SOFI-image of the n-th order are calculated from the values of the pixel time series in the form of a n-th order cumulant, whereas the final value assigned to a pixel can be imagined as the integral over a correlation function. The finally assigned pixel value intensities are a measure of the brightness and correlation of the fluorescence signal. Mathematically, the n-th order cumulant is related to the n-th order correlation function, but exhibits some advantages concerning the resulting resolution of the image. Since in SOFI several emitters per DLA are allowed, the photon count at each pixel results from the superposition of the signals of all activated nearby emitters. The cumulant calculation now filters the signal and leaves only highly correlated fluctuations. This provides a contrast enhancement and therefore a background reduction for good measure. As it is implied in the figure on the left the fluorescence source distribution: ∑ k = 1 N δ ( r → − r → k ) ⋅ ε k ⋅ s k ( t ) {\displaystyle \sum _{k=1}^{N}\delta ({\vec {r}}-{\vec {r}}_{k})\cdot \varepsilon _{k}\cdot s_{k}(t)} is convolved with the system's point spread function (PSF) U(r). Hence the fluorescence signal at time t and position r → {\displaystyle {\vec {r}}} is given by F ( r → , t ) = ∑ k = 1 N U ( r → − r → k ) ⋅ ε k ⋅ s k ( t ) . {\displaystyle F({\vec {r}},t)=\sum _{k=1}^{N}U({\vec {r}}-{\vec {r}}_{k})\cdot \varepsilon _{k}\cdot s_{k}(t).} Within the above equations N is the amount of emitters, located at the positions r → k {\displaystyle {\vec {r}}_{k}} with a time-dependent molecular brightness ε k ⋅ s k {\displaystyle \varepsilon _{k}\cdot s_{k}} where ε k {\displaystyle \varepsilon _{k}} is a variable for the constant molecular brightness and s k ( t ) {\displaystyle s_{k}(t)} is a time-dependent fluctuation function. The molecular brightness is just the average fluorescence count-rate divided by the number of molecules within a specific region. For simplification it has to be assumed that the sample is in a stationary equilibrium and therefore the fluorescence signal can be expressed as a zero-mean fluctuation: δ F ( r → , t ) = F ( r → , t ) − ⟨ F ( r → , t ) ⟩ t {\displaystyle \delta F({\vec {r}},t)=F({\vec {r}},t)-\langle F({\vec {r}},t)\rangle _{t}} where ⟨ ⋯ ⟩ t {\displaystyle \langle \cdots \rangle _{t}} denotes time-averaging. The auto-correlation here e.g. the second-order can then be described deductively as follows for a certain time-lag τ {\displaystyle \tau } : δ F ( r → , t ) = ⟨ δ F ( r → , t + τ ) ⋅ δ F ( r → , t ) ⟩ t {\displaystyle \delta F({\vec {r}},t)=\langle \delta F({\vec {r}},t+\tau )\cdot \delta F({\vec {r}},t)\rangle _{t}} From these equations it follows that the PSF of the optical system has to be taken to the power of the order of the correlation. Thus in a second-order correlation the PSF would be reduced along all dimensions by a factor of 2 {\displaystyle {\sqrt {2}}} . As a result, the resolution of the SOFI-images increases according to this factor. === Cumulants versus correlations === Using only the simple correlation function for a reassignment of pixel values, would ascribe to the independency of fluctuations of the emitters in time in a way that no cross-correlation terms would contribute to the new pixel value. Calculations of higher-order correlation functions would suffer from lower-order correlations for what reason it is superior to calculate cumulants, since all lower-order correlation terms vanish. == Cumulant-calculation == === Auto-cumulants === For computational reasons it is convenient to set all time-lags in higher-order cumulants to zero so that a general expression for the n-th order auto-cumulant can be found: A C n ( r → , τ 1 … n − 1 = 0 ) = ∑ k = 1 N U n ( r → − r → k ) ε k n w k ( 0 ) {\displaystyle AC_{n}({\vec {r}},\tau _{1\ldots n-1}=0)=\sum _{k=1}^{N}U^{n}({\vec {r}}-{\vec {r}}_{k})\varepsilon _{k}^{n}w_{k}(0)} w k {\displaystyle w_{k}} is a specific correlation based weighting function influenced by the order of the cumulant and mainly depending on the fluctuation properties of the emitters. Albeit there is no fundamental limitation in calculating very high orders of cumulants and thereby shrinking the FWHM of the PSF there are practical limitations according to the weighting of the values assigned to the final image. Emitters with a higher molecular brightness will show a strong increase in terms of the pixel cumulant value assigned at higher-orders as well as this performance can be expected from a diverse appearance of fluctuations of different emitters. A wide intensity range of the resulting image can therefore be expected and as a result dim emitters can get masked by bright emitters in higher-order images:. The calculation of auto-cumulants can be realized in a very attractive way in a mathematical sense. The n-th order cumulant can be calculated with a basic recursion from moments K n ( r → ) = μ n ( r → ) − ∑ i = 1 n − 1 ( n − 1 i ) K n − i ( r → ) μ i ( r → ) {\displaystyle K_{n}({\vec {r}})=\mu _{n}({\vec {r}})-\sum _{i=1}^{n-1}{\begin{pmatrix}n-1\\i\end{pmatrix}}K_{n-i}({\vec {r}})\mu _{i}({\vec {r}})} where K is a cumulant of the index's order, likewise μ {\displaystyle \mu } represents the moments. The term within the brackets indicates a binomial coefficient. This way of computation is straightforward in comparison with calculating cumulants with standard formulas. It allows for the calculation of cumulants with only little time of computing and is, as it is well implemented, even suitable for the calculation of high-order cumulants on large images. === Cross-cumulants === In a more advanced approach cross-cumulants are calculated by taking the information of several pixels into account. Cross-cumulants can be described as follows: C C n ( r → , τ 1 … n − 1 = 0 ) = ∏ j < l n U ( r → j − r → l n ) ⋅ ∑ i = 1 N U n ( r → i − ∑ k n r → k n ) ε i n w i ( 0 ) {\displaystyle CC_{n}({\vec {r}},\tau _{1\ldots n-1}=0)=\prod _{j

Feng Office Community Edition

Feng Office Community Edition (formerly OpenGoo) is an open-source collaboration platform developed and supported by Feng Office and the OpenGoo community. It is a fully featured online office suite with a similar set of features as other online office suites, like Google Workspace, Microsoft 365, Zimbra, LibreOffice Online and Zoho Office Suite. The application can be downloaded and installed on a server. Feng Office could also be categorized as collaborative software and as personal information manager software. == Features == Feng Office Community Edition main features include project management, document management, contact management, e-mail and time management. Text documents and presentations can be created and edited online. Files can be uploaded, organized and shared, independent of file formats. Organization of the information in Feng Office Community Edition is done using workspaces and tags. The application presents the information stored using different interfaces such as lists, dashboards and calendar views. == Licensing == Feng Office Community Edition is distributed under the GNU Affero General Public License, version 3 only. == Technology used == Feng Office uses PHP, JavaScript, AJAX (ExtJS) and MySQL technology. Several open source projects served as a basis for development. ActiveCollab's last open sourced release was used as the initial code base. It includes CKEditor for online document editing. == System requirements == The server could run on any operating system. The system needs the following packages: Apache HTTP Server 2.0+ PHP 5.0+ MySQL 4.1+ (InnoDB support recommended) On the client side, the user is only required to use a modern Web browser. == History == OpenGoo started as a degree project at the faculty of Engineering of the University of the Republic, Uruguay. The project was presented and championed by Software Engineer Conrado Viña. Software Engineers Marcos Saiz and Ignacio de Soto developed the first prototype as their thesis. Professors Eduardo Fernández and Tomas Laurenzo served as tutors. Conrado, Ignacio and Marcos founded the OpenGoo community and remain active members and core developers. The thesis was approved with the highest score. In 2008, Viña joined the Uruguayan software development company Moove It. Currently there is a second project for OpenGoo at the same university being developed by students Fernando Rodríguez, Ignacio Vázquez and Juan Pedro del Campo. Their project aims to build an open source Web-based spreadsheet. In December 2009 the OpenGoo name was changed to Feng Office Community Edition.

Scikit-learn

scikit-learn (formerly scikits.learn and also known as sklearn) is a free and open-source machine learning library for the Python programming language. It features various classification, regression and clustering algorithms including support-vector machines, random forests, gradient boosting, k-means and DBSCAN, and is designed to interoperate with the Python numerical and scientific libraries NumPy and SciPy. Scikit-learn is a NumFOCUS fiscally sponsored project. == Overview == The scikit-learn project started as scikits.learn, a Google Summer of Code project by French data scientist David Cournapeau. The name of the project derives from its role as a "scientific toolkit for machine learning", originally developed and distributed as a third-party extension to SciPy. The original codebase was later rewritten by other developers. In 2010, contributors Fabian Pedregosa, Gaël Varoquaux, Alexandre Gramfort and Vincent Michel, from the French Institute for Research in Computer Science and Automation in Saclay, France, took leadership of the project and released the first public version of the library on February 1, 2010. In November 2012, scikit-learn as well as scikit-image were described as two of the "well-maintained and popular" scikits libraries. In 2019, it was noted that scikit-learn is one of the most popular machine learning libraries on GitHub. At that time, the project had over 1,400 contributors and the documentation received 42 million visits in 2018. According to a 2022 Kaggle survey of nearly 24,000 respondents from 173 countries, scikit-learn was identified as the most widely used machine learning framework. == Features == Large catalogue of well-established machine learning algorithms and data pre-processing methods (i.e. feature engineering) Utility methods for common data-science tasks, such as splitting data into train and test sets, cross-validation and grid search Consistent way of running machine learning models (estimator.fit() and estimator.predict()), which libraries can implement Declarative way of structuring a data science process (the Pipeline), including data pre-processing and model fitting == Examples == Fitting a random forest classifier: == Implementation == scikit-learn is largely written in Python, and uses NumPy extensively for high-performance linear algebra and array operations. Furthermore, some core algorithms are written in Cython to improve performance. Support vector machines are implemented by a Cython wrapper around LIBSVM; logistic regression and linear support vector machines by a similar wrapper around LIBLINEAR. In such cases, extending these methods with Python may not be possible. scikit-learn integrates well with many other Python libraries, such as Matplotlib and plotly for plotting, NumPy for array vectorization, Pandas dataframes, SciPy, and many more. == History == scikit-learn was initially developed by David Cournapeau as a Google Summer of Code project in 2007. Later that year, Matthieu Brucher joined the project and started to use it as a part of his thesis work. In 2010, INRIA, the French Institute for Research in Computer Science and Automation, got involved and the first public release (v0.1 beta) was published in late January 2010. The project released its first stable version, 1.0.0, on September 24, 2021. The release was the result of over 2,100 merged pull requests, approximately 800 of which were dedicated to improving documentation. Development continues to focus on bug fixes, efficiency and feature expansion. The latest version, 1.8, was released on December 10, 2025. This update introduced native Array API support, enabling the library to perform GPU computations by directly using PyTorch and CuPy arrays. This version also included bug fixes, improvements and new features, such as efficiency improvements to the fit time of linear models. == Applications == Scikit-learn is widely used across industries for a variety of machine learning tasks such as classification, regression, clustering, and model selection. The following are real-world applications of the library: === Finance and Insurance === AXA uses scikit-learn to speed up the compensation process for car accidents and to detect insurance fraud. Zopa, a peer-to-peer lending platform, employs scikit-learn for credit risk modelling, fraud detection, marketing segmentation, and loan pricing. BNP Paribas Cardif uses scikit-learn to improve the dispatching of incoming mail and manage internal model risk governance through pipelines that reduce operational and overfitting risks. J.P. Morgan reports broad usage of scikit-learn across the bank for classification tasks and predictive analytics in financial decision-making. === Retail and E-Commerce === Booking.com uses scikit-learn for hotel and destination recommendation systems, fraudulent reservation detection, and workforce scheduling for customer support agents. HowAboutWe uses it to predict user engagement and preferences on a dating platform. Lovely leverages the library to understand user behaviour and detect fraudulent activity on its platform. Data Publica uses it for customer segmentation based on the success of past partnerships. Otto Group integrates scikit-learn throughout its data science stack, particularly in logistics optimization and product recommendations. === Media, Marketing, and Social Platforms === Spotify applies scikit-learn in its recommendation systems. Betaworks uses the library for both recommendation systems (e.g., for Digg) and dynamic subspace clustering applied to weather forecasting data. PeerIndex used scikit-learn for missing data imputation, tweet classification, and community clustering in social media analytics. Bestofmedia Group employs it for spam detection and ad click prediction. Machinalis utilizes scikit-learn for click-through rate prediction and relational information extraction for content classification and advertising optimization. Change.org applies scikit-learn for targeted email outreach based on user behaviour. === Technology === AWeber uses scikit-learn to extract features from emails and build pipelines for managing large-scale email campaigns. Solido applies it to semiconductor design tasks such as rare-event estimation and worst-case verification using statistical learning. Evernote, Dataiku, and other tech companies employ scikit-learn in prototyping and production workflows due to its consistent API and integration with the Python ecosystem. === Academia === Télécom ParisTech integrates scikit-learn in hands-on coursework and assignments as part of its machine learning curriculum. == Awards == 2019 Inria-French Academy of Sciences-Dassault Systèmes Innovation Prize: Awarded in recognition of scikit-learn's impact as a major free software breakthrough in machine learning and its role in the digital transformation of science and industry. 2022 Open Science Award for Open Source Research Software: Awarded by the French Ministry of Higher Education and Research as part of the second National Plan for Open Science. The project was recognized in the "Community" category for its technical quality, its large international contributor network, and the quality of its documentation.

Amazon Elastic Compute Cloud

Amazon Elastic Compute Cloud (EC2) is a part of Amazon's cloud-computing platform, Amazon Web Services (AWS), that allows users to rent virtual computers on which to run their own computer applications. EC2 encourages scalable deployment of applications by providing a web service through which a user can boot an Amazon Machine Image (AMI) to configure a virtual machine, which Amazon calls an "instance", containing any software desired. A user can create, launch, and terminate server-instances as needed, paying by the second for active servers – hence the term "elastic". EC2 provides users with control over the geographical location of instances that allows for latency optimization and high levels of redundancy. In November 2010, Amazon switched its own retail website platform to EC2 and AWS. == History == Amazon announced a limited public beta test of EC2 on August 25, 2006, offering access on a first-come, first-served basis. Amazon added two new instance types (Large and Extra-Large) on October 16, 2007. On May 29, 2008, two more types were added, High-CPU Medium and High-CPU Extra Large. There were twelve types of instances available. Amazon added three new features on March 27, 2008: static IP addresses, availability zones, and user-selectable kernels. On August 20, 2008, Amazon added Elastic Block Store (EBS). This provides persistent storage, a feature that had been lacking since the service was introduced. Amazon EC2 went into full production when it dropped the beta label on October 23, 2008. On the same day, Amazon announced the following features: a service level agreement for EC2, Microsoft Windows in beta form on EC2, Microsoft SQL Server in beta form on EC2, plans for an AWS management console, and plans for load balancing, autoscaling, and cloud monitoring services. These features were subsequently added on May 18, 2009. Amazon EC2 was developed mostly by a team in Cape Town, South Africa led by Chris Pinkham. Pinkham provided the initial architecture guidance for EC2 and then built the team and led the development of the project along with Willem van Biljon. == Instance types == Initially, EC2 used Xen virtualization exclusively. However, on November 6, 2017, Amazon announced the new C5 family of instances that were based on a custom architecture around the KVM hypervisor, called Nitro. Each virtual machine, called an "instance", functions as a virtual private server. Amazon sizes instances based on "Elastic Compute Units". The performance of otherwise identical virtual machines may vary. On November 28, 2017, AWS announced a bare-metal instance, a departure from exclusively offering virtualized instance types. As of January 2019, the following instance types were offered: General Purpose: A1, T3, T2, M5, M5a, M4, T3a Compute Optimized: C5, C5n, C4 Memory Optimized: R5, R5a, R4, X1e, X1, High Memory, z1d Accelerated Computing: P3, P2, G3, F1 Storage Optimized: H1, I3, D2 As of April 2018, the following payment methods by instance were offered: On-demand: pay by the hour without commitment. Reserved: rent instances with one-time payment receiving discounts on the hourly charge. Spot: bid-based service: runs the jobs only if the spot price is below the bid specified by bidder. The spot price is claimed to be supply-demand based, however a 2011 study concluded that the price was generally not set to clear the market, but was dominated by an undisclosed reserve price. In 2025, AWS expanded EC2 with the compute-optimized C8gn family, powered by Graviton4 and offering up to 600 Gbit/s network bandwidth (about 30% higher compute performance than C7gn), and introduced G6f fractional-GPU instances that let customers provision one-eighth, one-quarter, or one-half of an NVIDIA L4 GPU for right-sized graphics/ML workloads. === Cost === As of April 2018, Amazon charged about $0.0058 per hour ($4.176 per month) for the smallest "Nano Instance" (t2.nano) virtual machine running Linux or Windows. Storage-optimized instances cost as much as $4.992 per hour (i3.16xlarge). "Reserved" instances can go as low as $2.50 per month for a three-year prepaid plan. The data transfer charge ranges from free to $0.12 per gigabyte, depending on the direction and monthly volume (inbound data transfer is free on all AWS services). EC2 costs can be analyzed using the Amazon Cost and Usage Report. There are many different cost categories for EC2 including: hourly Instance Charges, Data Transfer, EBS Volumes, EBS Volume Snapshots, and Nat Gateway. === Free tier === As of December 2010 Amazon offered a bundle of free resource credits to new account holders. The credits are designed to run a "micro" sized server, storage (EBS), and bandwidth for one year. Unused credits cannot be carried over from one month to the next. === Reserved instances === Reserved instances enable EC2 or RDS service users to reserve an instance for one or three years. The corresponding hourly rate charged by Amazon to operate the instance is 35 to 75% lower than the rate charged for on-demand instances. Reserved instances can be purchased with three different payment options: All Upfront, Partial Upfront and No Upfront. The different purchase options allow for different structuring of payment models, with a larger discount given to customers that pay their reservation upfront. Reserved Instances are purchased based on a resource commitment. These reservations are made based on an instance type and a count of that instance type. For example, you could reserve 100 i3.large instances for a 3-year term. In September 2016, AWS announced several enhancements to Reserved instances, introducing a new feature called scope and a new reservation type called a Convertible. In October 2017, AWS announced the allowance to subdivide the instances purchased for more flexibility. === Spot instances === Cloud providers maintain large amounts of excess capacity they have to sell or risk incurring losses. Amazon EC2 Spot instances are spare compute capacity in the AWS cloud available at up to 90% discount compared to On-Demand prices. As a trade-off, AWS offers no SLA on these instances and customers take the risk that it can be interrupted with only two minutes of notification when Amazon needs the capacity back. Researchers from the Israeli Institute of Technology found that "they (Spot instances) are typically generated at random from within a tight price interval via a dynamic hidden reserve price". Some companies, like Spotinst, are using machine learning to predict spot interruptions up to 15 minutes in advance. === Savings Plans === In November 2019, Amazon announced Savings Plans. Savings Plans are an alternative to Reserved Instances that come in two different plan types: Compute Savings Plans and EC2 Instances Savings Plans. Compute Savings Plans allow an organization to commit to EC2 and Fargate usage with the freedom to change region, family, size, availability zone, OS and tenancy inside the lifespan of the commitment. EC2 Instance Savings plans provide a larger discount than Compute Savings Plans but are less flexible meaning a user must commit to individual instance families within a region to take advantage, but with the freedom to change instances within the family in that region. AWS uses the Cost Explorer to automatically calculate recommendations for the commitments you should make how that commitment will look like as a monthly charge on your AWS bill. AWS Savings Plans are purchased based on hourly spend commitment. This hourly commitment is made using the discounted pricing of the savings plan you are purchasing. For example, you could commit to spending $5 per hour, on a Compute Savings Plan, for a 3-year term. == Features == === Operating systems === When it launched in August 2006, the EC2 service offered Linux and later Sun Microsystems' OpenSolaris and Solaris Express Community Edition. In October 2008, EC2 added the Windows Server 2003 and Windows Server 2008 operating systems to the list of available operating systems. In March 2011, NetBSD AMIs became available. In November 2012, Windows Server 2012 support was added. Since 2006, Colin Percival, a FreeBSD developer and Security Officer, solicited Amazon to add FreeBSD. In November 2012, Amazon officially supported running FreeBSD in EC2. The FreeBSD/EC2 platform is maintained by Percival who also developed the secure deduplicating Amazon S3-cloud based backup service Tarsnap. Amazon has their own Linux distribution based on Fedora and Red Hat Enterprise Linux as a low cost offering known as the Amazon Linux AMI. Version 2013.03 included: Linux kernel, Java OpenJDK Runtime Environment and GNU Compiler Collection. On November 30, 2020, Amazon announced that it would be adding macOS to the EC2 service. Initial support was announced for macOS Mojave and macOS Catalina running on Mac Mini. === Managed Container and Kubernetes Services === Amazon Elastic Container Registry (ECR) is a Docker registry service for Amazon EC2

15.ai

15.ai was a free non-commercial web application and research project that uses artificial intelligence to generate text-to-speech voices of fictional characters from popular media. Created by a pseudonymous artificial intelligence researcher known as 15, who began developing the technology as a freshman during their undergraduate research at the Massachusetts Institute of Technology (MIT), the application allows users to make characters from video games, television shows, and movies speak custom text with emotional inflections. The platform is able to generate convincing voice output using minimal training data; the name "15.ai" references the creator's statement that a voice can be cloned with just 15 seconds of audio. It was an early example of an application of generative artificial intelligence during the initial stages of the AI boom. Launched in March 2020, 15.ai became an Internet phenomenon in early 2021 when content utilizing it went viral on social media and quickly gained widespread use among Internet fandoms, such as the My Little Pony: Friendship Is Magic, Team Fortress 2, and SpongeBob SquarePants fandoms. The service featured emotional context through emojis, precise pronunciation control, and multi-speaker capabilities. Critics praised 15.ai's accessibility and emotional control but criticized its technical limitations in prosody options and non-English language support, with mixed results depending on character complexity. 15.ai is credited as the first platform to popularize AI voice cloning in memes and content creation. Voice actors and industry professionals debated 15.ai's implications, raising concerns about employment impacts, voice-related fraud, and potential misuse. In January 2022, it was discovered that a company called Voiceverse had generated voice lines using 15.ai without attribution, promoted them as the byproduct of their own technology, and sold them as non-fungible tokens (NFT) without permission. News publications universally characterized this incident as the company having "stolen" from 15.ai. The service went offline in September 2022 due to legal issues surrounding artificial intelligence and copyright. Its shutdown was followed by the emergence of commercial alternatives whose founders have acknowledged 15.ai's pioneering influence in the field of deep learning speech synthesis. On May 18, 2025, 15 launched 15.dev as the sequel to 15.ai. == History == === Background === The field of speech synthesis underwent a significant transformation with the introduction of deep learning approaches. In 2016, DeepMind's publication of the WaveNet paper marked a shift toward neural network-based speech synthesis, which enabled higher audio quality via causal convolutional neural networks. Previously, concatenative synthesis—which worked by stitching together pre-recorded segments of human speech—was the predominant method for generating artificial speech, but it often produced robotic-sounding results at the boundaries of sentences. In 2018, Google AI's Tacotron 2 showed that neural networks could produce highly natural speech synthesis but required substantial training data (typically tens of hours of audio) to achieve acceptable quality. When trained on two hours of training data, the output quality degraded while still being able to maintain intelligible speech; with 24 minutes of training data, Tacotron 2 failed to produce intelligible speech. The same year saw the emergence of HiFi-GAN, a generative adversarial network (GAN)-based vocoder that improved the efficiency of waveform generation while producing high-fidelity speech, followed by Glow-TTS, which introduced a flow-based approach that allowed for both fast inference and voice style transfer capabilities. Chinese tech companies like Baidu and ByteDance also made contributions to the field by developing breakthroughs that further advanced the technology. === 2016–2020: Conception and development === 15.ai was conceived in 2016 as a research project in deep learning speech synthesis by a developer known as 15 (at the age of 18) during their freshman year at MIT as part of its Undergraduate Research Opportunities Program. 15 was inspired by DeepMind's WaveNet paper, with development continuing through their studies as Google AI released Tacotron 2 the following year. By 2019, they had demonstrated at MIT their ability to replicate WaveNet and Tacotron 2's results using 75% less training data than previously required. The name "15.ai" is a reference to the developer's statement that a voice can be cloned with as little as 15 seconds of data. 15 had originally planned to pursue a PhD based on their undergraduate research, but opted to work in the tech industry instead after their startup was accepted into the Y Combinator accelerator in 2019. After their departure in early 2020, 15 returned to their voice synthesis research and began implementing it as a web application. According to a post on X from 15, instead of using conventional voice datasets like LJSpeech that contained simple, monotone recordings, they sought out more challenging voice samples that could demonstrate the model's ability to handle complex speech patterns and emotional undertones. During this phase, 15 discovered the Pony Preservation Project, a collaborative project started by /mlp/, the My Little Pony board on 4chan. Contributors of the project had manually trimmed, denoised, transcribed, and emotion-tagged thousands of voice lines from My Little Pony: Friendship Is Magic and had compiled them into a dataset that provided ideal training material for 15.ai. === 2020–2022: Release and operation === 15.ai was released on March 2, 2020 as a free and non-commercial web application that did not require user registration to use, but did require the user to accept its terms of service before proceeding. At the time of its launch, the platform had a limited selection of available characters, including those from My Little Pony: Friendship Is Magic and Team Fortress 2. Users were permitted to create any content with the synthesized voices under two conditions: they had to properly credit 15.ai by including "15.ai" in any posts, videos, or projects using the generated audio; and they were prohibited from mixing 15.ai outputs with other text-to-speech outputs in the same work to prevent misrepresentation of the technology's capabilities. On March 8, 2020, Tyler McVicker of Valve News Network uploaded a YouTube video showcasing 15.ai. More voices were added to the website in the following months. In late 2020, 15 implemented a multi-speaker embedding in the deep neural network, which enabled the simultaneous training of multiple voices. Following this, the website's roster expanded from eight to over fifty characters. In addition, this implementation allowed the deep learning model to recognize common emotional patterns across different characters, even when certain emotions were missing from the characters' training data. By May 2020, the site had served over 4.2 million audio files to users. In early 2021, the application gained popularity after skits, memes, and fan content created using 15.ai went viral on Twitter, TikTok, Reddit, Twitch, Facebook, and YouTube. At its peak, the platform incurred operational costs of US$12,000 per month from AWS infrastructure needed to handle millions of daily voice generations; despite receiving offers from companies to acquire 15.ai and its underlying technology, the website remained independent and was funded out of the personal previous startup earnings of the developer. === 2022: Voiceverse NFT controversy === On January 14, 2022, 15 discovered that a blockchain-based company called Voiceverse had generated voice lines using 15.ai, falsely showcased them on Twitter as a demonstration of their own voice technology without permission or attribution, and sold them as NFTs. This came shortly after 15 had stated in December 2021 that they had no interest in incorporating NFTs into their work. A screenshot of the log files posted by 15 showed that Voiceverse had generated audio of characters from My Little Pony: Friendship Is Magic using 15.ai and pitched them up to make them sound unrecognizable, a violation of 15.ai's terms of service, which explicitly prohibited commercial use and required proper attribution. When confronted with evidence, Voiceverse stated that their marketing team had used 15.ai without proper attribution while rushing to create a demo. In response, 15 tweeted "Go fuck yourself," which went viral, amassing hundreds of thousands of retweets and likes on Twitter in support of the developer. The tweets showcasing the stolen voices were subsequently deleted. ==== Aftermath ==== The controversy raised concerns about NFT projects, which, according to critics, were frequently associated with intellectual property theft and questionable business practices. The incident was documented in the AI Incident Database (AIID) and the AI, Alg

Microsoft Teams

Microsoft Teams is a team collaboration platform developed by Microsoft as part of the Microsoft 365 suite. It offers features such as workspace chat, video conferencing, file storage, and integration with both Microsoft and third-party applications and services. Teams gradually replaced earlier Microsoft messaging and collaboration platforms, including Skype for Business, Skype, Flip, and Microsoft Classroom. The platform saw significant growth during the COVID-19 pandemic, alongside competitors such as Zoom, Slack, and Google Meet, as organizations shifted to remote work and virtual meetings. As of January 2023, Microsoft reported approximately 280 million monthly active users. == History == On August 29, 2007, Microsoft acquired Parlano, the developer of the persistent group chat tool MindAlign. Years later, on March 4, 2016, Microsoft considered acquiring Slack for $8 billion. However, the proposal was reportedly opposed by Bill Gates, who advocated for focusing on enhancing Skype for Business instead. Lu Qi, then executive vice president of Applications and Services, had led the initiative to pursue the Slack acquisition. Following Lu's departure later that year, Microsoft announced Microsoft Teams on November 2, 2016, at an event in New York City, positioning it as a direct competitor to Slack. Teams launched worldwide on March 14, 2017. The service was initially led by corporate vice president Brian MacDonald. In response to the launch, Slack published a full-page advertisement in The New York Times welcoming the competition and outlining its product philosophy. Although Slack was used by 28 companies in the Fortune 100, The Verge wrote that executives would question paying for the service if Teams provides a similar function in their company's existing Office 365 subscription. However, ZDNET noted that the platforms initially served different markets, as Teams did not support external users, making it less appealing to small businesses and freelancers, a limitation Microsoft later addressed. In response to Teams' announcement, Slack deepened in-product integration with Google services. In May 2017, Microsoft announced that Teams would replace Microsoft Classroom in Office 365 Education. A free version of Teams was released on July 12, 2018, offering most core features at no cost, albeit with limits on users and storage. In January 2019, Microsoft introduced updates targeting "Firstline Workers" to improve Teams’ performance across shared or limited-access devices. In September 2019, Microsoft announced the retirement of Skype for Business in favor of Teams, which took effect on July 31, 2021. In early 2020, Microsoft introduced a push-to-talk "Walkie Talkie" feature aimed at firstline workers using smartphones and tablets over Wi-Fi or cellular networks. The COVID-19 pandemic significantly boosted usage of Teams. On March 19, 2020, Microsoft reported 44 million daily active users. In April, the platform logged 4.1 billion meeting minutes in a single day. A public preview of Microsoft Teams for Linux was released in December 2019, but the Linux client was discontinued in 2022. In July 2020, Microsoft shut down its video game livestreaming platform Mixer, and announced that some of its technologies would be repurposed for use in Teams. On February 28, 2025, Microsoft announced that Skype would be fully retired on May 5, 2025, with users given options to export their data or transition to Microsoft Teams. In October 2025, together with other Microsoft 365 suite apps, Teams had its logo updated. == Usage == == Underlying software == Microsoft Teams, as part of the Microsoft 365 suite, utilizes SharePoint and Exchange Online. Each Team, Shared Channel, and Private Channel has its own Microsoft 365 Group and SharePoint Site used for file storage. Messages are stored in Cosmos DB and are journaled to Exchange Online mailboxes. Private messages, including messages in Private Channels, are journaled to the sender and recipients' mailboxes. Public Channel messages are journaled to their corresponding Team's group mailbox, whereas, messages from Shared Channels are journaled to their own mailboxes. Contacts and voicemail are stored in Exchange Online. Microsoft Teams client is a web-based desktop app, originally developed on top of the Electron framework which combines the Chromium rendering engine and the Node.js JavaScript platform. Version 2.0 client was rebuilt using the Evergreen version of Microsoft Edge WebView2 in place of Electron. == Features == === Chats === Teams allows users to communicate in two-way persistent chats with one or multiple participants. Participants can message using text, emojis, stickers and gifs, as well as sharing links and files. In August 2022, the chat feature was updated for "chat with yourself"; allowing for the organization of files, notes, comments, images, and videos within a private chat tab. === Teams === Teams allows communities, groups, or teams to contribute in a shared workspace where messages and digital content on a specific topic are shared. Team members can join through an invitation sent by a team administrator or owner or sharing of a specific URL. Teams for Education allows admins and teachers to set up groups for classes, professional learning communities (PLCs), staff members, and everyone. === Channels === Channels allow team members to communicate without the use of email or group SMS (texting). Users can reply to posts with text, images, GIFs, and image macros. Direct messages send private messages to designated users rather than the entire channel. Connectors can be used within a channel to submit information contacted through a third-party service. Connectors include Mailchimp, Facebook Pages, Twitter, Power BI and Bing News. === Group conversations === Ad-hoc groups can be created to share instant messaging, audio calls (VoIP), and video calls inside the client software. === Telephone replacement === A feature on one of the higher cost licencing tiers allows connectivity to the public switched telephone network (PSTN) telephone system. This allows users to use Teams as if it were a telephone, making and receiving calls over the PSTN, including the ability to host "conference calls" with multiple participants. === Meeting === Meetings can be scheduled with multiple participants able to share audio, video, chat and presented content with all participants. Multiple users can connect via a meeting link. Automated minutes are possible using the recording and transcript features. Teams has a plugin for Microsoft Outlook to schedule a Teams Meeting in Outlook for a specific date and time and invite others to attend. If a meeting is scheduled within a channel, users visiting the channel are able to see if a meeting is in progress. ==== Teams Live Events ==== Teams Live Events replaces Skype Meeting Broadcast for users to broadcast to 10,000 participants on Teams, Yammer, or Microsoft Stream. ==== Breakout Rooms ==== Breakout rooms split a meeting into small groups. This is often utilized for collaboration during trainings or any environment where having all participants speak at once could be disruptive or unfeasible. Breakout rooms can be set by the hosts to a certain length of time, after which all participants will automatically rejoin the main meeting room. ==== Front Row ==== Front Row adjusts the layout of the viewer's screen, placing the speaker or content in the center of the gallery with other meeting participant's video feeds reduced in size and located below the speaker. === Education === Microsoft Teams for Education allows teachers to distribute, provide feedback, and grade student assignments turned in via Teams using the Assignments tab through Office 365 for Education subscribers. Quizzes can also be assigned to students through an integration with Office Forms. === Protocols === Microsoft Teams is based on a number of Microsoft-specific protocols. Video conferences are realized over the protocol MNP24, known from the Skype consumer version. VoIP and video conference clients based on SIP and H.323 need special gateways to connect to Microsoft Teams servers. With the help of Interactive Connectivity Establishment (ICE), clients behind Network address translation routers and restrictive firewalls are also able to connect, if peer-to-peer is not possible. === Integrations === Microsoft Teams has integrations through Microsoft AppSource, its integration marketplace. In 2020, Microsoft partnered with KUDO, a cloud-based solution with language interpretation, to allow integrated language meeting controls. In June 2022, an update was released using AI to improve call audio through the elimination of background feedback loops and cancelling non-vocal audio. == Anti-trust controversy == In July 2023, the European Commission opened an anti-trust investigation into the possibility that Microsoft unfairly used its office suite market power to increase sales of Teams and hurt

Amazon Kinesis

Amazon Kinesis is a family of services provided by Amazon Web Services (AWS) for processing and analyzing real-time streaming data at a large scale. Launched in November 2013, it offers developers the ability to build applications that can consume and process data from multiple sources simultaneously. Kinesis supports multiple use cases, including real-time analytics, log and event data collection, and real-time processing of data generated by IoT devices. == History == Amazon Kinesis was launched by Amazon Web Services (AWS) in November 2013 as a managed service for processing and analyzing real-time streaming data at a large scale. The service was introduced to address the growing need for businesses to process and analyze data as it was generated, rather than in batches, allowing for real-time insights and decision-making. Since its launch, the Amazon Kinesis family of services has expanded to include four main components: Kinesis Data Streams, Kinesis Data Firehose, Kinesis Data Analytics, and Kinesis Video Streams. Each of these components serves a specific purpose in the processing and analysis of real-time streaming data. In August 2015, AWS announced the availability of Kinesis Data Firehose, a fully managed service for delivering real-time streaming data to destinations such as Amazon S3, Amazon Redshift, and Amazon Elasticsearch. A year later in August 2016, AWS launched Kinesis Data Analytics, enabling customers to analyze streaming data in real time using standard SQL queries. AWS introduced Kinesis Video Streams, a fully managed service for securely capturing, processing, and storing video streams for analytics and machine learning applications, was introduced by AWS in November 2017. == Components == Amazon Kinesis is composed of four main services: Kinesis Data Streams, Kinesis Data Firehose, Kinesis Data Analytics, and Kinesis Video Streams. === Kinesis Data Streams === Kinesis Data Streams is a scalable and durable real-time data streaming service that captures and processes gigabytes of data per second from multiple sources. It enables the storage and processing of data in real time, making it useful for applications that require immediate insights, such as monitoring and alerting. === Kinesis Data Firehose === Kinesis Data Firehose is a fully managed service for delivering real-time streaming data to destinations such as Amazon S3, Amazon Redshift, Amazon Elasticsearch, and AWS-partner data stores. With Data Firehose, users can configure and scale data delivery without manual intervention. === Kinesis Data Analytics === Kinesis Data Analytics enables the analysis of streaming data in real time using standard SQL or Apache Flink. === Kinesis Video Streams === Kinesis Video Streams is a fully managed service for securely capturing, processing, and storing video streams for analytics and machine learning. It supports multiple video codecs and streaming protocols, making it suitable for various use cases, such as security and surveillance, video-enabled IoT devices, and live event broadcasting. == Integration == Amazon Kinesis can be easily integrated with other AWS services, such as AWS Lambda, Amazon S3, Amazon Redshift, and Amazon OpenSearch. This integration enables developers to build end-to-end streaming data processing applications, taking advantage of the extensive AWS ecosystem. == Use cases == Some common use cases for Amazon Kinesis include: Real-time analytics: Analyzing streaming data in real time to provide immediate insights and make data-driven decisions. Log and event data collection: Collecting, processing, and analyzing log and event data generated by applications, infrastructure, and devices. IoT data processing: Processing and analyzing large volumes of data generated by IoT devices in real time. Machine learning: Ingesting and processing video streams for machine learning applications, such as object recognition, facial recognition, and sentiment analysis. == Pricing == Amazon Kinesis follows a pay-as-you-go pricing model, with costs depending on the chosen service, data volume, and processing power required. AWS provides a free tier for Kinesis Data Streams and Kinesis Data Firehose, allowing users to get started with the services at no cost.